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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Mol Biochem Parasitol. 2010 May 19;173(2):97–106. doi: 10.1016/j.molbiopara.2010.05.009

Table 1.

Primer sequences

Primer Name Primer sequence 5′-3′
SmPAL-F1 CGGCACGAGGGAACCAT CATACC
SmPAL-R1 ACTACT ACGTCCCGTTGGT TTAC
SmPAL-Xba1-F TCTAGAAT GTACTTTACATTAATAATC
SmPAL-Bgl2-R AGATCTATAATCGTATTCAA AGTTAAAACG
SmPAL-Nhe1-F GCTAGCATCGTAT ATGATTTCC
SmPAL-Hpa1-R GAATTCGTTAACTCACG CAGG
SmPAL-ISH-T7-F1 TAATACGACTCACTATAGGGCATGTATCGTATATGATTTCC
SmPAL-ISH-R1 GAGATTTAAATCATATGGTCC
SmPAL-ISH-F2 CATGTATCGTATATGATTTCC
SmPAL-ISH-T7-R2 TAATACGACTCACTAT AGGGGAGATTTAAATCATATGGTCC
SmPAL-RNAi-T7-F1 TAATAC GACTCACTATAGGGCATGTATCGTATATGATTTCC
SmPAL-RNAi-R1 GTATTT TCATCCCATACTCG
SmPAL-RNAi-F2 CATGTATCGTATA TGATTTC
SmPAL-RNAi-T7-R2 TAATACGACTCACTATAGGGGTATTTTCATCCC ATACTCG
SmPHM-RNAi-T7-F1 TAATACGACTCACTATAGGGCGTATTCCAGGAGTGACAAC
SmPHM-RNAi-R1 GACTCAGATTCACGACATATAG
SmPHM-RNAi-F2 CGTATTCCAGGAGTGACAAC
SmPHM-RNAi-T7-R2 TAATACGACTCACTATAGGGGACTCAGATTCACGACATATAG
SmPAL-RT S1 GCTTTAAACCTGGTTCAAGC 1322 bp RT-PCR product
SmPAL-RT AS1 ATAATCGTATTCAAAGTTAAAACG
SmAP-RT S1 GCCATCCGACAAGGAATATAAGTGT 98 bp RT-PCR product
SmAP-RT AS1 GGTCCATTGAAAAAGGAGGATATGAGA

T7 RNA polymerase promoter sequences are underlined