Fig. 5.

Demonstration of PEA induced the cleavage of caspase-3, capase-6, DFF45, and lamin A. (A) Pro-form of caspase-3 (32 kDa) was degraded, and 17 kDa and 12 kDa cleaved forms were produced after PEA treatment. Caspase-6 pro-enzyme (34 kDa) was degraded, and 16 kDa cleaved forms were produced after PEA treatment. (B) Three cleaved products of DFF45 (30, 20, and 11 kDa) were demonstrated in YD-9 cells treated with PEA, which are evident in later time points (48 and 72 hours). Two cleaved products of lamin A (45 and 28 kDa) are demonstrated in YD-9 cells treated with PEA, which are shown at 24 hours after PEA treatment.