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. Author manuscript; available in PMC: 2011 Mar 20.
Published in final edited form as: Tetrahedron. 2010 Mar 20;66(12):2175–2180. doi: 10.1016/j.tet.2010.01.053

Table I.

Initial rates and enantioselectivities for the product formation in the transesterification reaction of sec-alcohols with vinyl butyrate for different preparations of subtilisin C

Substrates Enzyme
Preparation		 VSa,b,c Enantioselectivityc,d
graphic file with name nihms-172780-t0007.jpg Lyophilized
MβCD		 1
14		 93
126
graphic file with name nihms-172780-t0008.jpg Lyophilized
MβCD		 5
59		 93
141
graphic file with name nihms-172780-t0009.jpg Lyophilized
MβCD		 7
228		 82
104
graphic file with name nihms-172780-t0010.jpg Lyophilized
MβCD		 1
32		 148
240
a

Initial rates for the S-enantiomer in nmol mg−1min−1

b

Enzyme concentration 1mg/mL in all experiments

c

Experiments were performed in duplicate. Results are the average of both trials.

d

Measured by the ratio of [kcat/KM]S/[kcat/KM]R = VS[R]/VR[S].