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. Author manuscript; available in PMC: 2010 Jul 23.
Published in final edited form as: Trends Cell Biol. 2007 Sep 4;17(8):377–382. doi: 10.1016/j.tcb.2007.08.001

Figure 2. Rings, bracelets, sleeves and chevrons formed by kinetochore proteins.

Figure 2

A. Dam1 complex rings around microtubules. Recombinant Dam1 complex bound to paclitaxel-stabilized microtubule, Images are electron micrographs of negative stained material. Bar represents 50 nm. Reprinted from (8) with permission from Nature.

B. MCAK stabilized tubulin bracelets. Paclitaxel stabilized microtubule in the presence of recombinant MCAK (core motor domain plus 64 residues of the neck domain) and AMPPNP. Image is an electron micrograph of negative stained material. Bar represents 50 nm. Reprinted from (5) with permission from Landes.

C. HURP-induced tubulin sheets. Recombinant HURP was incubated with tubulin in the presence of GTP. Vitrification by quick-freezing was followed by freeze-drying and then unidirectional metal shadowing before cryo elecron microscopy. The red box indicates a region of the microtubule covered with a sleeve. Bar represents 50 nm. Reprinted from (9) with permission from Elsevier.

D. Ndc80 chevrons bound to microtubules. Recombinant Ndc80 complex bound to microtubules stabilized with GMPCPP. Image is an electron micrograph of negative stained material. Bar represents 50 nm. Reprinted from (10) with permission from Elsevier.