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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Hepatology. 2010 Apr;51(4):1291–1301. doi: 10.1002/hep.23471

Figure 5.

Figure 5

Effect of MafG, c-Maf or double knockdown on Nrf2 nuclear binding to ARE and ARE-dependent promoter activity. A) HuH-7 cells were treated with scrambled (SC), MafG, c-Maf or combined MafG and c-Maf siRNA for 24 hours followed by LCA treatment for another 20 hours. EMSA and supershift analysis was done as described in Methods using antibodies to Nrf2, MafG and c-Maf. B) HuH-7 cells were treated with the same siRNAs as above for 24 hours and then transfected with ARE promoter construct containing native or mutated sequences as described in Methods. Results represent mean±SE from 3 independent experiments done in triplicates. *p<0.05 vs. scrambled, **p<0.005 vs. scrambled, †p<0.05 vs. MafG siRNA.