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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Hepatology. 2010 Apr;51(4):1291–1301. doi: 10.1002/hep.23471

Figure 6.

Figure 6

Effect of MafG, c-Maf or double knockdown on expression of GSH synthetic enzymes and GSH levels. HuH-7 cells were treated with MafG (A and C) or c-Maf (B and D) siRNA for up to 72 hours and processed for Northern (A and B) or Western (C and D) analyses as described in Methods. In E), HuH-7 cells were treated with scrambled (SC), MafG, c-Maf, or both siRNA for 48 hours and processed for Western blot analyses for GCLC, GCLM and GS. In F), GSH levels were measured following the same treatments as in E. Results are mean±SE from 3 to 4 independent determinations. *p<0.001 vs. SC, †p<0.05 vs. MafG or c-Maf siRNA alone.