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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Hepatology. 2010 Apr;51(4):1291–1301. doi: 10.1002/hep.23471

Figure 8.

Figure 8

Effects of in vivo MafG and c-Maf knockdown in BDL mice on gene expression, ARE nuclear binding and GSH levels. Mice were subjected to BDL or sham surgery and treated with lentivirus carrying scrambled (SC), MafG, c-Maf or both MafG and c-Maf siRNAs as described in Methods and sacrificed on day 14. Parts A and B show Western blot analyses of MafG, c-Maf, Nrf2, GCLC, GCLM and GS. Part C shows EMSA and supershift analysis for Nrf2 binding to ARE using nuclear proteins from BDL mice treated with SC, MafG, c-Maf or both siRNAs. Part D summarizes hepatic GSH levels in these mice. Results are mean±SE from 3 to 4 mice for each condition. *p<0.001 vs. Sham+SC, **p<0.05 vs. Sham+SC, †p<0.005 vs. BDL+SC, ††p<0.001 vs. BDL+SC and BDL+either Maf siRNA.