TABLE 2.
Rates [nmol/min/(nmol of P450)] determined for phenacetin metabolism by CYP1A2 WT and mutantsa
| CYP1A2 | NADPH Oxidized | O2 Consumed | Product Formed | H2O2 Produced | Excess H2Ob |
|---|---|---|---|---|---|
| WT | 43 ± 4 | 22.7 ± 0.9 | 1.28 ± 0.08 | 14 ± 2 | 15 ± 2 |
| T124S | 27 ± 2 | 14.8 ± 0.4 | 0.29 ± 0.03 | 10 ± 0 | 9 ± 1 |
| T223N | 41 ± 3 | 17.3 ± 0.7 | 0.30 ± 0.00 | 10 ± 1 | 14 ± 2 |
| V227G | 40 ± 3 | 16.9 ± 0.5 | 0.38 ± 0.02 | 9 ± 1 | 15 ± 2 |
| N312L | 32 ± 2 | 18.3 ± 0.3 | 0.31 ± 0.00 | 13 ± 2 | 10 ± 1 |
| L382V | 84 ± 6 | 47.0 ± 1.3 | 3.03 ± 0.06 | 29 ± 3 | 30 ± 4 |
| L382V/T223N | 98 ± 7 | 57.9 ± 2.5 | 3.91 ± 0.10 | 38 ± 3 | 32 ± 5 |
| L382V/N312L | 70 ± 4 | 41.0 ± 2.0 | 3.05 ± 0.15 | 29 ± 3 | 18 ± 3 |
| L382V/T223N/N312L | 72 ± 5 | 41.7 ± 1.1 | 2.18 ± 0.03 | 28 ± 3 | 23 ± 3 |
| L382V/T124S/N312L | 42 ± 3 | 34.2 ± 0.9 | 3.67 ± 0.10 | 26 ± 2 | 9 ± 2 |
a
Data are means of triplicate determinations.
b
Excess water (H2O) was calculated from the equation: H2O = NADPH − H2O2 − product.