TABLE 3.

Effect of mutations on coupling efficiency of CYP1A2 at different branching points of P450 cycle^a

CYP1A2	Product/NADPHb	H2O2/O2c	H2O/Productd
WT	0.030	0.62	11.72
T124S	0.011	0.68	31.03
T223N	0.007	0.58	46.67
V227G	0.010	0.53	39.47
N312L	0.010	0.71	32.26
L382V	0.036	0.62	9.9
L382V/T223N	0.040	0.66	8.18
L382V/N312L	0.044	0.71	5.9
L382V/T223N/N312L	0.030	0.67	10.55
L382V/T124S/N312L	0.087	0.76	2.45

^a Ratios were calculated from parameters in Table 2.

^b Efficiency of coupling reducing equivalents to product.

^c Effect on uncoupling at first or second branch point.

^d Effect on uncoupling at third branch point.