Figure 1. RhoU mRNA expression is defective in Stat3−/− MEFs.
Total RNA was extracted from Stat3+/+, Stat3−/− and revertant (rev) MEFs either untreated or stimulated with IL-6 or OSM for the indicated times. RhoU mRNA was measured by qRT-PCR. Expression was normalized to 18S, and results are shown as means ± S.E.M. of two independent experiments performed in duplicate. Asterisks indicate statistically significant differences between selected groups (*P < 0.05 and **P < 0.01). A.U., arbitrary units.