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. Author manuscript; available in PMC: 2010 Jul 23.
Published in final edited form as: Biochem J. 2009 Jun 26;421(2):283–292. doi: 10.1042/BJ20090061

Figure 4. RhoU induction downstream of Wnt-1 is independent from STAT3 and β-catenin.

Figure 4

(a) Stat3+/+ MEFs were transfected with the RhoU −1300 and −770 vectors, either wild-type or mutated (M) in the indicated LEF/TCF or STAT3-binding sequences. Wnt-1 stimulation and data analysis were performed as described for Figure 3. (b) The RhoU −770 construct and TOPFlash and FOPFlash reporters were co-transfected with a stable mutant β-catenin (S33A) or with an empty control vector in Stat3 wild-type MEFs. Luciferase activity was normalized and reported as above. A.U., arbitrary units.