Table 1. Transplantation results of Tumor Cell Populations.
| Number of cells transplanted | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| <100 | 100-1K | 1K-5K | 5K-10K | 10K-100K | >100K | ||||
| Tumor Genotype | Cell Type | Number of mice with tumors / Number transplanted (%) |
Frequency of TPCs* | p-value | |||||
| Kras; p53-flox | Sca1+Cells | 3/5 (60%) |
2/3 (67%) |
10/14 (71%) |
2/2 (100%) |
ND | ND | 1/1489 | 0.0006† |
| Sca1-Cells | ND | 2/9 (22%) |
6/17 (35%) |
2/4 (50%) |
ND | ND | 1/7567 | ||
| Kras | Sca1+Cells | ND | 1/4 (25%) |
3/10 (30%) |
5/11 (45%) |
5/8 (63%) |
ND | 1/13990 | 0.7311‡ |
| Sca1-Cells | ND | 1/3 (33%) |
4/8 (50%) |
14/18 (78%) |
4/6 (67%) |
7/8 (88%) |
1/16037 | ||
Varied numbers of tumor cells as indicated were transplanted in sterile PBS into Foxn1nu (nude) recipients.
The number of recipient mice with at least one secondary tumor was determined by histological analysis.
Results shown are the combination of 14 and 9 different transplantation experiments with Kras and Kras;p53-flox tumor cells, respectively.
See also Tables S1 and S2 for more details.
*
TPCs: Tumor-propagating cells. The frequency of cells with tumor-propagating capacity in the population was determined by limiting dilution analysis with L-calc software using the transplanted cell numbers shown in Tables S1 and S2.
†
Kras;p53-flox Sca1 + cells vs. Kras;p53-flox Sca1- cells.
‡
Kras Sca1 + cells vs. Kras Sca1- cells.
ND: not determined.