Table 1.
Select list of transcripts up-regulated 90 min after relAL.p. induction.
| Gene Name / Function | Gene.ID | Annotation | ppGpp0 prelAL.p./pempty |
|---|---|---|---|
| Flagellar Genes | |||
| fleQ | lpg0853 | Master regulator of flagellar gene transcription (Class I) | 2.01 |
| fliH | lpg1758 | Export and assembly (Class IIa) | 2.41 |
| fliG | lpg1759 | Motor switch protein (Class IIa) | 3.88 |
| fleR | lpg1762 | Two-component response regulator (Class IIa) | 3.54 |
| fleS | lpg1763 | Two-component sensor histidine kinase (Class IIa) | 2.58 |
| fliN | lpg1791 | Motor switch protein (Class IIa) | 5.25 |
| flgB | lpg1216 | Proximal rod protein (Class IIb) | 4.32 |
| flgC | lpg1217 | Proximal rod protein (Class IIb) | 4.03 |
| flgG | lpg1221 | Distal rod protein (Class IIb) | 4.81 |
| flgH | lpg1222 | L-ring protein precursor (Class IIb) | 4.42 |
| flgJ | lpg1224 | Peptidoglycan-hydrolyzing protein (Class IIb) | 2.87 |
| fleN | lpg1783 | Regulator of flagellar synthesis (Class IIb) | 3.01 |
| flhF | lpg1784 | Biosynthesis regulator GTP-binding protein (Class IIb) | 3.87 |
| Dot/Icm Structural Genes | |||
| icmR | lpg0443 | Chaperone for IcmQ; a pore forming molecule | 2.46 |
| icmL/dotI | lpg0449 | Inner membrane protein | 2.23 |
| icmK/dotH | lpg0450 | Might be an outer membrane channel | 2.18 |
| icmE/dotG | lpg0451 | Major component of a channel | 1.96 |
| icmG/dotF | lpg0452 | Interacts with substrates; predicted channel component | 2.14 |
| icmC/dotE | lpg0453 | Predicted inner-membrane protein, Similar to DotV | 2.31 |
| icmD/dotP | lpg0454 | Predicted inner-membrane protein | 3.43 |
| icmJ/dotN | lpg0455 | Probable ATPase component | 2.10 |
| Dot/Icm Secreted Effectors | |||
| legC3 | lpg1701 | Coiled-coil domain, disrupts vacuolar trafficking, IcmSW-dependent | 2.11 |
| ylfA | lpg2298 | Coiled-coil domain, disrupts vacuolar trafficking, | 3.33 |
| sdeD | lpg2509 | Substrate of the Dot/Icm system | 1.98 |
| lepA | lpg2793 | SNAREs and coiled-coil domain; bacterial egress | 3.99 |
| Regulatory Factors | |||
| lpg0433 | lpg0433 | Putative transcriptional regulator | 1.99 |
| letE | lpg0537 | Transmission trait enhancer protein | 3.60 |
| rpoS | lpg1284 | RNA polymerase sigma factor (sigma-38) | 3.11 |
| relA | lpg1457 | ppGpp synthetase | 16.16 |
| lpg2732 | lpg2732 | Putative response regulator | 2.28 |
| rsmZ | non-coding, CsrA binding, regulatory RNA | 4.18 | |
| Known Virulence Factors | |||
| mip | lpg0789 | Macrophage infectivity potentiator | 2.06 |
| katB | lpg2389 | Catalase-peroxidase | 3.03 |
| enhC | lpg2639 | Enhanced entry protein | 2.73 |
| Fatty Acid / Carbon Metabolism | |||
| plsC | lpg0551 | similar to 1-acyl-sn-glycerol-3-phosphate acyltransferase | 2.51 |
| phaB1 | lpg0560 | Similar to acetoacetyl-CoA reductase | 2.09 |
| yfcX | lpg1596 | similar to alpha subunit of fatty-acid oxidation complex | 3.25 |
| phbC3 | lpg2260 | Similar to poly(3-hydroxyalkanoate) synthetase | 2.25 |
| pta | lpg2261 | Similar to phosphotransacetylase | 2.12 |
| pksJ | lpg2186 | Similar to polyketide synthase of type I | 2.17 |
| lpg2837 | lpg2837 | phospholipase/lecithinase/hemolysin, lysophospholipase A | 6.13 |
The data were collected from two independent biological replicates. See Fig. S1 for qRT-PCR validation. p < 0.01 for all targets shown.