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. Author manuscript; available in PMC: 2010 Jul 23.
Published in final edited form as: Biotechnol J. 2010 Feb;5(2):163–171. doi: 10.1002/biot.200900111

Figure 2.

Figure 2

Comparison of ISOkine Flt3 ligand and an E. coli produced Flt3 ligand from commercial source. Lane 1 contains a molecular weight marker, lane 2, purified ISOkine Flt3 ligand and in lane 3 E. coli produced Flt3 ligand from commercial source. Figure A shows Coomassie blue stained SDS-PAGE gel. Six hundred nanogram was loaded on the gel. Figure B–D show Western blot analysis using polyclonal anti-Flt3 ligand antibodies (B), anti-α-1,3-fucose specific antibody (C) and anti-β-1,2-xylose specific antibody (D). Two hundred and fifty nanogram of protein was loaded on the gel for the Western blot application.