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. Author manuscript; available in PMC: 2010 Jul 23.
Published in final edited form as: Hum Mutat. 2009 Mar;30(3):411–416. doi: 10.1002/humu.20879

Fig. 2.

Fig. 2

The effect of SEPN1 SRE mutations on UGA redefinition. A) Percent readthrough of the UGA codon in the wildtype (WT) or mutant SRE contexts (c.1388G>T, c.1397G>A, c.1405C>T, and c.1406G>A) were measured using the 2luc reporter system in vitro using a RRL transcription and translation system supplemented with increasing amounts of SBP2-CT (SBP2) at the indicated concentrations. B) Percent readthrough of the UGA codon in the wildtype (WT) or mutant SRE context was measured following expression of the 2luc reporter constructs in transfected 293FT cells.