Skip to main content
. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Hum Mutat. 2010 Aug;31(8):924–929. doi: 10.1002/humu.21297

Figure 1.

Figure 1

A: Holo-TC uptake in skin fibroblasts in culture. Cells were seeded at 0.2 × 106 cells/2 ml in six-well plates overnight. For uptake of holo-TC, [57Co]cyanocobalamin-TC (0.03 pmol) was added in 1ml DMEM and incubated at 37°C. Following removal of the medium and washing, the cells were detached by incubating with 0.5 ml 0.05% trypsin/EDTA. The radioactivity in the trypsin/EDTA solution and the cells was determined as a measure of TCblR-mediated binding and uptake of holo-TC. B: The kinetics of holo-TC binding to TCblR was determined by incubating 1.5 × 106 cells with 0.03 to 0.24 pmol of holo-TC for 1 hr at room temperature. Inset shows the Scatchard analysis of the binding data. C: TCblR mRNA in fibroblasts from the patient (WG3733) and two control cell lines (MCH 064 and MCH 065) as determined by qPCR.