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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Hum Mutat. 2010 Aug;31(8):924–929. doi: 10.1002/humu.21297

Figure 3.

Figure 3

Accumulation of homocysteine (Hcy) (A) and methylma-lonic acid (MMA) (B) in culture medium of fibroblasts grown in the presence of increasing concentrations of holo-TC. Bovine recombinant holo-TC was added to 2ml culture medium containing 0.2 × 106 cells. The medium collected after 8 days of culture was used for the determination of Hcy and MMA. Significant increases in Hcy at 0 and 140 pmol/l and MMA at 0–14,800 pmol/l were observed in the medium from the patient’s fibroblasts (WG3733) compared to a control cell line under identical culture conditions. The statistical analysis was performed using the Microcal Origin v7.0 software. The independent Student t-test with a confidence interval of 95% was used to establish significant differences.