Table 3.

TMC1 polymorphisms that do not segregate within families

Exon	Nucleotide Change
1	g.74G>A (-467 from the ATG)
3	g.[236 -67G>A (+) 322A>G (-219 from the ATG)]
4	g.346 -11delT
6	c.45C>T (synonymous)
8	c.237 -44G>A
8	c.241G>A (p.E81K) *
10	c.[535 +101A>G (+) 535 +106_113delCAAACAAA]
14	c.1029 +85T>C
16	c.1404 +32A>G
17	c.1457T>C (p.M486T) †
22	c.2208 +22A>G

^*p.E81K does not segregate with the HI status in three families. Additionally p.E81K is at the intracellular glutamic acid (E)-rich region and is non-conserved.

^†p.M486T was found in the heterozygous state in two hearing individuals from two different families but not among the hearing-impaired. The residue is predicted to be intracellular and is non-conserved.