Table 2.
Twenty four FBS and human serum HS and CS mono-, di-, tri-, tetra-, and hexasaccharides.
| Heparan sulfate | ||||
|---|---|---|---|---|
| (HS Disaccharide %. B:FBS, H:human serum) | Observed ions | Elution time | TIC and % of TIC |
|
| FBS | Human | |||
| ΔUA-GlcNAc (B46.6%, H37.5%) | z1 378.12 | 3.9 | 1822 18.80% |
990 14.10% |
| ΔUA-GlcNS (B20.9%, H25.5%) | z1 416.08, z2 207.53 | 9 | 2128 22.00% |
1787 25.40% |
| ΔUA-GlcNAc6S (B8.5%, H9.6%) | z1 458.09, z2 228.54 | 8.7 | 852 8.80% |
673 9.60% |
| ΔUA2S-GlcNAc (B1.2%, H1.6%) | z1 458.09, z2 228.54 | 8.7 | 135 1.40% |
112 1.60% |
| ΔUA-GlcNS6S (B5.4%, H9.6%) | z1 496.04, z2 247.51 | 14.2 | 1048 10.80% |
1214 17.30% |
| ΔUA2S-GlcNS (B8.2%, H8.5%) | z1 496.04, z2 247.51 | 14.2 | 1610 16.50% |
1081 15.40% |
| ΔUA2S-GlcNS6S (B9.2%, H7.6%) | z1 576.01, z2 287.49 z3 191.32 | 19.3 | 2105 21.70% |
1167 16.60% |
| Chondroitin sulfate | ||||
| (CS Disaccharide %. B:FBS, H:human serum) | ||||
| ΔUA-GalNAc (B6.3%, H2.8%) | z1 378.12 | 3.7 | 13906 2.50% |
54000 5.50% |
| ΔUA-GalNAc6S (B7.2%, H10.0%) | z1 458.03, z2 228.53 | 7.8 | 4154 6.80% |
81663 8.30% |
| ΔUA-GalNAc4S (B83.7%, H85.0%) | z1 458.03, z2 228.53 | 7.8 | 48992 80.20% |
693647 70.50% |
| ΔUA2S-GalNAc (B1.5%, H2.2%) | z1 458.03, z2 228.53 | 7.8 | 855 1.40% |
17710 1.80% |
| ΔUA-GlcNAc+S2 (B1.3%, H0.02%) | z1 538.06, z2 268.51,z3 178.67 | 14.9 | 14428 2.60% |
1239 0.13% |
| GalNAc+S1 | z1 300.05 | 3.8 | 6674 1.20% |
2165 0.22% |
| GalNAc+S2 | z1 380.02, z2 189.50 | 9 | 2750 0.46% |
186 0.02% |
| GlcA/IdoA-GalNAc+S1 | z1 476.10, z2 237.53 | 7.2 | 677 0.12% |
520 0.05% |
| ΔUA-GalNAc-UA-ol | z1 556.07 | 7.3 | 2658 0.48% |
259 0.26% |
| ΔUA-GalNAc4S-UA-ol | z1 636.15, z2 317.56 | 13.5 | 2311 0.42% |
319 0.03% |
| ΔUA-GalNAc-UA-GalNAc-ol | z1 839.26, z2 419.11 | 14.1 | 1190 0.21% |
88 0.01% |
| GalNAc-UA-GalNAc+S3 | z2 419.05, z3 279.03 | 19.3 | 1604 0.29% |
149 0.02% |
| ΔUA-GalNAc-GlcA-Gal-Gal-Xylol | z2 505.67 | 7.1 | 550 0.10% |
0 0 |
| ΔUA-GalNAc-GlcA-Gal-Gal-Xylol+S1 | z2 545.63, z3 363.42 | 13 | 2539 0.46% |
371 0.04% |
| ΔUA-GalNAc-GlcA-Gal-Gal-Xylol+S2 | z2 585.63, z3 390.08 | 18.6 | 14007 2.52% |
125000 12.70% |
| ΔUA-GalNAc-GlcA-Gal-Gal-Xylol+S3 | z2 690.2, z3 416.74 | 20.2 | 282 0.05% |
1817 0.18% |
| ΔUA-GalNAc-GlcA-Gal-Gal-Xylol+S2+Sialic acid | z2 731.22, z3 487.12, z4 365.08 | 20.1 | 208 0.04% |
4277 0.44% |