Figure 4. Dendritic cells are not required for IFN-I production induced by TMPD.

A) Flow cytometry of peritoneal DCs after TMPD treatment. Box indicate CD11c⁺ I-A⁺ DCs. B) Depletion in CD11c-DTR mice 2 days after diphtheria toxin (DT) injection. Box indicates CD11c⁺ I-A⁺ DCs. C) Quantification of peritoneal dendritic cells and Ly6C^hi monocytes. D) Quantification of splenic DC depletion. MDCs were defined as CD11c^hi I-A⁺ CD11b⁺ cells and PDCs were defined as CD11c⁺ B220⁺ PDCA-1⁺. E) IFN-I expression (conventional PCR) and F) ISG expression (RT-PCR) in peritoneal exudates cells. Each bar represents the mean of 6 animals and error bars indicate s.d. * p < 0.05 (Student’s t-test).