Table 1. Monosaccharide composition of contaminated heparin lots.
Twenty-six suspect heparin samples were acid hydrolyzed and analyzed chemically for galactosamine, glucosamine, and uronic acid content. The recovery of galactosamine and glucosamine relative to uronic acid was used to determine the relative amount of chondroitin/dermatan sulfate and heparin/heparan sulfate, respectively.
| Contaminated heparin | Galactosamine Content (%) | Uronic acid (% of heparin) |
|---|---|---|
| LSCH1 | 2 ± 1.4 | 114 ± 6% |
| LSCH2 | 8.0 ± 1.0 | 114 ± 7% |
| LSCH3 | 9.6 ± 0.6 | 108 ± 7% |
| LSCH4 | 10.5 ± 2.1 | 124 ± 3% |
| LSCH5 | 13.8 ± 4.1 | 115 ± 3% |
| LSCH6 | 15.7 ± 0.6 | 122 ± 3% |
| OSCH1 | 10.5 ± 1.5 | 100 ± 20% |
| OSCH2 | 13.0 ± 1.0 | 109 ± 1% |
| OSCH3 | 13.9 ± 0.6 | 110 ± 10% |
| OSCH4 | 23.6 ± 1.5 | 108 ± 0.7% |
| OSLSCH1 | 9.6 ± 0.1 | 110 ± 13% |
| OSLSCH2 | 11.0 ± 0.4 | 100 ± 16% |
| OSLSCH3 | 18.9 ± 0.8 | 110 ± 23% |
| OSLSCH4 | 21.0 ± 1.2 | 110 ± 16% |
| OSLSCH5 | 22.8 ± 1.4 | 120 ± 13% |
| OSLSCH6 | 23.3 ± 0.6 | 107 ± 3% |
| OSLSCH7 | 23.7 ± 0.2 | 90 ± 10% |
| OSLSCH8 | 29.1 ± 0.8 | 101 ± 3% |
| OSLSCH9 | 29.3 ± 3.5 | 108 ± 0.7% |
| OSLSCH10 | 37.1 ± 0.14 | 90 ± 10% |
| S1 | 2.5 ± 0.6 | 92 ± 5% |
| S2 | 3.5 ± 0.5 | 95 ± 3% |
| S3 | 3.8 ± 1.6 | 102 ± 3% |
| G1 | 8.7 ± 2.1 | 100 ± 2% |
| G2 | 7.3 | 110 ± 2% |
| G3 | 6.5 ± 0.6 | 107 ± 8% |
The individual lots were analyzed for uronic acid, galactosamine and glucosamine after acid hydrolysis. The recovery of each hexosamine was used to determine the relative amount of chondroitin/dermatan sulfate and heparin/heparan sulfate, respectively.
Abbreviations: LSCH, low-sulfated contaminant containing heparin; OSCH, oversulfated contaminant containing heparin; OSLSCH, both oversulfated and low-sulfated contaminant containing heparin; S (suspected) lots passed both CE and NMR screen; G (Germany) lots passed screening by NMR but not by CE.