Table 3. Detection of oversulfated heparan sulfate in contaminated heparin by liquid chromatography/mass spectrometry.
Proposed structures | A. Total Ion Current |
|||
---|---|---|---|---|
Elution time (min) | OSCH2 | LSCH4 | Ratio | |
DA+0S | 15.6 | 7559 | 13000 | 0.58 |
DH+1S | 17.0 | 453 | 846 | 0.54 |
DH+3S | 20.1 | 0 | 37000 | |
DAUH+4S | 23.3 | 0 | 1092 | |
DHUH+6S | 24.7 | 0 | 1675 | |
DAUA+1S+103 | 25.8 | 0 | 4885 | |
DAUH+3S+103 | 28.0 | 0 | 1003 |
DA refers to a disaccharide containing an Δ4,5-unsaturated uronic acid (U) linked to N-acetylglucosamine (A). UAUH refers to a tetrasaccharide composed of uronic acid (U, GlcA or IdoA)-N-acetylglucosamine (A)-uronic acid (U)-glucosamine (H). S refers to sulfate.