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. 2010 Jul 23;5(7):e11640. doi: 10.1371/journal.pone.0011640

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Deere et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Comparison of RT-SHIV RNA loads determined using the standard virus load assay (SVLA) or the ultracentrifugation virus load assay (UVLA) from macaque 35940, a macaque that was taken off HAART to allow for viral rebound. Triplicate assays with triplicate TaqMan RT-PCR for UVLA, single TaqMan RT-PCR for the SVLA. Mean with 95% confidence intervals and the results of an unpaired t test. (B) Plasma RT-SHIV RNA analysis at necropsy of 5 HAART-treated macaques. Assays were done in duplicate with triplicate TaqMan RT-PCR except macaque 35342 which was sampled in triplicate with triplicate TaqMan RT-PCR. Mean with 95% confidence intervals. (C) FeLV RNA analysis of the samples displayed in Figure 2B. Cycle threshold (Ct) obtained from TaqMan RT-PCR for FeLV analyzed in duplicate. Mean with SD.