Table 1.
Transfected DNA source | Foci/μg DNA* | Transformation efficiency** | 95% confidence intervals |
---|---|---|---|
HCCR-1 | 13/15 | 0.9 | 11.2-13.8 |
Wild-type ras | 18/15 | 1.2 | 16.0-19.1 |
Vector alone | 0/15 | 0 | - |
*HEK-293cells (3 × 105 cells) were transfected with 5 μg of DNA obtained from indicated source. The results reported here were derived from 3 independent experiments.
**Transformed cell aggregates, foci, were counted by visual examination starting 2-3 weeks after transfection and the transformation efficiencies (i.e., the number of foci per microgram of DNA transfected) were determined.