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Chk1-Ser345 is activated by Vpr and HU/UV with different cell cycle outcomes. Synchronized G1/S HeLa cells by DT were treated with HU, UV or transduced with Adv-Vpr at time 0, collected at the indicated time, and then subjected to Western blot analysis (A) using anti-Chk1-Ser345 and anti-γH2AX-Ser139 antibodies. The cell cycle profiles of differently treated cells were analyzed at the indicated time after the DT release by flow cytometric analysis (B).
