Table 2.
Readthrough efficiencies from the mutant constructs
Test | Codon | Readthrough | Standard |
Sequnces | Frequency | Efficiency | Deviation |
CAGGLN UAG CAA | 3.41% | 0.77% | 0.08% |
CAAGLN UAG CAA | 1.18% | 2.01% | 0.80% |
CAUHIS UAG CAA | 0.97% | 0.06% | 0.03% |
CACHIS UAG CAA | 1.50% | 0.30% | 0.18% |
GGGGLY UAG CAA | 1.59% | 0.18% | 0.08% |
GGAGLY UAG CAA | 1.80% | 0.49% | 0.23% |
GGUGLY UAG CAA | 1.21% | 0.10% | 0.04% |
GGCGLY UAG CAA | 2.31% | 0.16% | 0.08% |
CAA UAG CAG | 0.43% | 0.05% | |
CAA UAG CAA | 2.01% | 0.80% | |
CAA UAG CAU | 0.07% | 0.04% | |
CAA UAG CAC | 0.066% | 0.05% | |
CAA UAG GGG | 0.022% | 0.01% | |
CAA UAG GGA | 0.028% | 0.01% | |
CAA UAG GGU | 0.033% | 0.01% | |
CAA UAG GGC | 0.053% | 0.03% |
(a) The last amino acid inserted before the stop is indicated together with the codon frequency compiled from Mus musculus [20]).