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. 2010 Jul 23;5(7):e11747. doi: 10.1371/journal.pone.0011747

Figure 1. The Pf fikk7.1 and Pf fikk12 genes were disrupted by a double-crossover event.

Figure 1

A. Schematic representation of the Pf fikk loci in the FCR3 wild type and FIKK-KO parasites. The plasmid constructs, pHTK-FIKK, contain two portions of the specific Pf fikk gene (dark grey portions), which were cloned in the same direction flanking the hdhfr cassette. The crosses indicate the two regions where homologous integration has occurred, here represented for Pf fikk12. The restriction sites are shown both for the wild type gene and the mutant gene. B. Southern blot analysis of the parental FCR3 strain and two representative clones of both FIKK12-KO (left panel) and FIKK7.1-KO (right panel). Hybridizations were carried out with 5' and 3' probes. The position of the probes is shown in A.