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. 2010 Jul 23;5(7):e11729. doi: 10.1371/journal.pone.0011729

Figure 5. PTK6 downregulation affects IGF-1 dependent signaling pathways.

Figure 5

A) Reverse-phase protein arrays (RPPA) reveal changes in IGF-1-stimulated signaling with PTK6 downregulation. RPPA analysis was performed on lysates of IGF-1R cells overexpressing Bcl2 (IGF-1R/Bcl2) that were transfected with control or PTK6 siRNA oligonucleotides in suspension cultures containing IGF-1 (100ng/ml). The heatmap represents values normalized to luciferase control from transfections performed in triplicate. B) shRNA-mediated downregulation of PTK6 in MCF-7 cells decreases ligand-stimulated IGF-1R phosphorylation. MCF-7 cells expressing control or PTK6 shRNA were stimulated in suspension cultures for the indicated times with 100ng/ml IGF-1. Lysates were probed with the indicated antisera. C) DOV-13 cells cultured on adherent plates were stimulated with 100ng/ml IGF-1 for 20 minutes. Lysates were probed with the indicated antisera. D) PTK6 downregulation inhibits IGF-1- stimulated activation of Akt and Erk/MAPK. Lysates of MCF-7 cells stimulated with the indicated concentrations of IGF-1 for 60 minutes (left panel) or 15 minutes (right panel) in suspension cultures were probed with the indicated antisera.