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. 2010 Jul 23;5(7):e11762. doi: 10.1371/journal.pone.0011762

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Suchard et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Lymphocytes were gated according to forward and side scatter. CD3 positive lymphocytes were selected. CD25 expression on the x-axis was plotted against CD4 on the y-axis. A gate was set according to CD25 expression on the CD4⁻ population. This gave a percentage of CD3⁺CD4⁺CD25⁺ lymphocytes as a percentage of CD3⁺ (figure in top-right quadrant). This percentage was then used to calculate the percentage of CD3⁺CD4⁺CD25⁺ lymphocytes as a percentage of the CD3⁺CD4⁺ population (CD4 population being top-left and right quadrants added together).