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. 2010 Jul 23;5(7):e11752. doi: 10.1371/journal.pone.0011752

Figure 4. Zymogram analysis of ADH isozymes from C. maltosa Xu316 and recombinant E. coli BL21(DE3).

Figure 4

Lane 1 in all figures and lane 2 in A and D: crude extracts from Xu316 cells grown for 18 h in YP medium containing 80 g/l xylose. Lane 3 in A, lane 2 and 3 in B, and lane 2 in C: crude extracts from E. coli BL21(DE3) overexpressing the recombinant CmADH1 (A), CmADH2A (B) and CmADH2B (C) without His-tags, respectively. D. Mixed crude extracts of the recombinant CmADH1 and CmADH2A. Amounts of proteins were given on the bottom of the lanes. Hypothetical isozyme composition of the major ADH bands in the electrophoretic pattern of C. maltosa was shown on the right of the lanes.