Figure 7. mRNA levels of three CmADH genes in wide type strain Xu316 (A) and their changes in ADH2A-deficient mutant M3-400 (B).

C. maltosa cells were grown in YP medium containing different carbon sources under aerobic conditions: 80 g/l glucose (D), 2% (V/V) ethanol (E), 2% (V/V) glycerol (G), and 80 g/l xylose (X). Cells were harvested in the mid-exponential phase (for 12 h in glucose medium, 16 h in ethanol or glycerol medium and 24 h in xylose medium) for quantitative real-time RT-PCR.