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. 2010 Jul 23;5(7):e11755. doi: 10.1371/journal.pone.0011755

Figure 3. Identification of PIP5Kγ as a SIRT1-binding protein.

Figure 3

(A) PIP5Ks were identified as SIRT1-associated protein by immunoprecipitation with anti-FLAG antibodies from HEK293T cells expressing FLAG-SIRT1. A full-length SIRT1 and inactive form of SIRT1 (H355A) interacted with PIP5K (+). (B) Immunoprecipitation analysis of endogenous SIRT1 and endogenous PIP5Kα, PIP5Kβ, PIP5Kγ, or PIP4Kβ in HEK293T cells. (C) Mapping the interaction interface of SIRT1 with PIP5Kγ. A schematic representation of the SIRT1 interaction site (right panel; positive (+), negative (−)).