Figure 3.

Quantitative analysis of ovarian follicles in newborn and pubertal mice. Ovaries from mice of the indicated ages and genotypes were serially sectioned, and all follicles from every fifth section were counted, categorized, and scored as healthy or atretic. One ovary (left) from each animal was evaluated. A) Ovaries of 5-d mice; n = 6/genotype. B) Ovaries of 42-d mice: primordial (a), primary (b), secondary (c), and antral follicles (d); n = 5 (Wnt4^flox/−;Amhr2^{tm3(cre)Bhr/+}), or n = 8 (Wnt4^flox/−). Data represent raw follicle count numbers and were not adjusted to estimate the total ovarian follicle population. All data are expressed as means ± se (columns and error bars). *P < 0.05, **P < 0.01 vs. control.