Figure 5.

Expression of gonadotropin receptors and hCG-responsive genes in the Wnt4^flox/−;Amhr2^{tm3(cre)Bhr/+} model. Quantitative RT-PCR analysis of the expression of the indicated genes in granulosa cells isolated from immature, eCG-treated mice of the indicated genotypes (n=4–5 animals/genotype/time point), either without (0h post hcG) or 12 h after the administration of an ovulatory dose of hCG (12h post hcG). All data were normalized to the housekeeping gene Rpl19 and are expressed as means ± se (columns and error bars). *P < 0.05 vs. control.