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. 2010 Aug;24(8):3010–3025. doi: 10.1096/fj.09-145789

Figure 6.

Figure 6

Regulation of established ovarian WNT/CTNNB1 pathway target genes by WNT4. A) Quantitative RT-PCR analysis of the expression of the indicated genes in cultured granulosa cells treated with the Ad-eGFP or Ad-Wnt4 adenovirus; n = 4 samples/treatment, performed in triplicate. B) Quantitative RT-PCR analysis of the expression of the indicated genes in granulosa cells isolated from immature, eCG-treated mice of the indicated genotypes (n=4–5 animals/genotype/time point), either without (0h post hCG) or 12 h after the administration of an ovulatory dose of hCG (12h post hCG). All data were normalized to the housekeeping gene Rpl19. C) Serum P4 and E2 levels in mice of the indicated genotypes. Samples were taken from immature, eCG-treated mice either without or 12 or 24 h after the administration of an ovulatory dose of hCG. Samples from adult mice were taken at 8 wk of age from random-cycling females; n = 4–10 samples/genotype/time point. All data are expressed as means ± se (columns and error bars). *P < 0.05 vs. control.