Figure 6.

SNX6 negatively regulates the retrograde transport of BACE1. A) BACE1 antibody uptake assay was performed using the cell lines used in Fig. 2A. Cells were transfected with BACE1. After 1 d of incubation, BACE1 at the cell surface was labeled with anti-BACE1 (N-terminal) antibody and subsequently chased for indicated time periods. Representative confocal images are shown. Scale bar = 10 μm. B) Quantitative analysis of BACE1 subcellular localization; 125 cells/case, on average, were examined for degree of perinuclear accumulation near the TGN. Categories used for scoring degree of accumulation: little, partial, prominent, and exclusive. C) Percentages of cells that showed dominant perinuclear accumulation (=prominent+exclusive) were plotted at each chase time (n=3). Values are means ± sd. *P < 0.05, **P < 0.001; 2-tailed t test.