Skip to main content
. 2010 Aug;24(8):3083–3092. doi: 10.1096/fj.09-152835

Figure 6.

Figure 6

Histone H3K4 trimethylation decreases with age in the promoter regions of age-down-regulated genes. Histone modifications were studied by ChIP followed by real-time PCR using chromatin isolated from 1 and 4 wk murine organs (5 animals/time point). A) Primers specific for 4 different regions −3 kb to +1 kb from the transcription start sites of Plagl1, Mdk, and Peg3 were used. ChIP was performed with antibodies against acetyl-H3K9/K14 (H3Ac), trimethyl-H3K4 (H3K4me3) or trimethyl-H3K27 (H3K27me3), and IgG (negative control). B) Primers specific for the promoter regions of 11 age-down-regulated genes, 5 age-up-regulated genes, and 3 controls (including chromosome 19 23.12 Mb, a genomic region distant from any promoters) were used. ChIP was performed with an antibody against H3K4me3 different from that in panel A. Results (means±se) were normalized to input (DNA before ChIP). *P < 0.05, **P < 0.01; ANOVA.