TABLE 1.
Primer sequences
| Gene symbol | Application | Forward primer, 5′→3′ | Reverse primer, 5′→3′ |
|---|---|---|---|
| BCL2 | qPCR | TATTGTGGCTGCACTTGCTC | TGTTGCCCAACTGCAAAATA |
| RORA | qPCR | ACACCAGCATCAGGCTTCTT | GGTCTGCCACGTTATCTGCT |
| BCL2 | Bisulfite sequencing Nested PCR-outer | GGTGGTTTAGAGGAGGGTTTTT | ACAAAAATCCTCTTCTAATTAAACTC |
| BCL2 | Bisulfite sequencing Nested PCR-inner | GAGAATGAAGTAAGAGGATAGGT | CACCCTTTCTCCTCCTCCTAATC |
| RORA | MSP: methylated | GGTTGGAGAAGTTTTCGTTAGC | GACGAACGAACAAACAAAAACG |
| MSP: unmethylated | TTGGTTGGAGAAGTTTTTGTTAGT | CAAACAAAAACACAAAAAAACACA |