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. 2010 Aug;24(8):3093–3102. doi: 10.1096/fj.10-155317

Figure 7.

Figure 7

IL-4 promotes the expression and phosphorylation of NR2B. A) Immunoblotting (top) and quantification (bottom) of NR2A and NR2B in the membrane-enriched fraction of the mouse hippocampus in non-Tg (NTg), uninjected APP+PS1 (uninj.), APP+PS1 mice injected with AAV-GFP (GFP), and APP+PS1 mice injected with AAV-IL-4 (IL-4). B) Immunoblotting (top) and quantification (bottom) of phospho-Tyr1472 NR2B (pNR2B) in the same fraction of the hippocampus. *P < 0.05, **P < 0.01, ***P < 0.001 vs. NTg group; #P < 0.05, ###P < 0.001 vs. AAV-GFP group; 1-way ANOVA and Newman-Keuls post hoc test. Error bars = se (n=6/group). C) Immunoblotting (top) and quantification (bottom) of pNR2B, NR2B, and β-actin in IL-4-treated primary cultured neurons. D) Immunoblotting (top) and quantification (bottom) of biotinylated NR2B at the cell surface level, total NR2B in IL-4 and/or oligomeric Aβ42-treated primary cultured neurons. **P < 0.01, ***P < 0.001 vs. control; ##P < 0.01 vs. Aβ42 group; 1-way ANOVA and Newman-Keuls post hoc test. Error bars represent se (n=3/group). Con, control; N.D., no significant difference.