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. 2009 Jun 30;122(15):2604–2612. doi: 10.1242/jcs.047183

Fig. 7.

Fig. 7.

Renal tubule polarity becomes dependent on the exocyst subunit Exo84 during late stages of development. (A-D) stage 11 wild-type (A,C) and exo84 mutant (B,D) embryos. (A,C) In wild-type embryos Crb (green, A), Baz (red, A) and Sas (red, C) localise apically in the renal tubules (arrows) and epidermis (arrowheads) and Fas II (green, C) localises to the lateral membrane of tubule cells. (B,D) In exo84 mutants the apical localisation of Crb, Baz and Sas is lost in epidermal cells (arrowheads), but is unaffected in the renal tubules and Fas II localises correctly to lateral tubule cell membranes (arrows). (E-H) Stage 16 wild-type (E,G) and exo84 mutant (F,H) tubules. (E,G) In wild-type tubules, Crb (E, green), Baz (E, red) and Sas (G, red) localise to apical cell surfaces and Fas II (G, green) concentrates laterally. (F,H) Stage 16 exo84 mutant tubules remain short and fat and the cells are irregular in shape (compare H with wild type in G). Apical proteins have spread into the lateral membranes (F, arrow) or are partially lost from the apical domains (compare H, with wild type in G, arrowheads). Although basolateral proteins localise as in wild type, the cells appear irregular and more rounded in shape (compare H with wild type in G).