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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Dev Biol. 2010 Apr 27;344(1):79–93. doi: 10.1016/j.ydbio.2010.04.018

Figure 8. Markers of definitive endoderm are not expressed in Klf6−/− ES cells.

Figure 8

Klf6+/+ and Klf6−/− ES cells were differentiated along the endoderm linage and RNA samples were obtained each day following activin addition to the EBs (see Figure S3). qPCR was used to detect Klf6 (a), Hnf3b (b), Cxcr4 (c), Sox17 (d), Gata4 (e) and compared to Gapdh as a reference. Levels were normalized to the expression in Klf6+/+ ES cells on day 2 of the differentiation protocol. Errors bars show standard deviation from three independent experiments.