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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Dev Biol. 2010 Apr 27;344(1):79–93. doi: 10.1016/j.ydbio.2010.04.018

Figure 9. Forced Klf6 expression enhances endoderm differentiation.

Figure 9

(a) Stable ES cells expressing Klf6 under a doxycyclin inducible promoter were cultured as EBs for 1 day, and then doxycycline was added, resulting in a 40% increase in Klf6 mRNA assessed by qPCR, using Gapdh as a reference (a). (b-d) Expression of Cxcr4, Sox17, Gata4 were detected in cells with and without doxycycline by qPCR and normalized to the expression level in cells without Klf6 induction (dox-). Errors bars show standard deviation from three independent experiments.