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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Dev Biol. 2010 May 10;344(1):248–258. doi: 10.1016/j.ydbio.2010.05.003

Fig. 4. Stage and tissue-specific expression pattern of Tcrk in T. castaneum determined by qRT-PCR.

Fig. 4

(A). Stage-specific expression pattern of Tcrk. The whole body samples were collected at one-day intervals during the final instar larval and pupal stages, and 3-day-old male and female (B). Tissue-specific expression pattern of Tcrk. The tissue samples were collected at 0 h after the pupation. Total RNA was extracted from pools of three larvae or pupae whole bodies, or tissues dissected from 6–7 beetles per treatment. The expression levels of Tcrk were normalized using Tcrp49 mRNA levels as an internal control. Mean + SE of three independent experiments are shown. Means with the same letter are not significantly different (α = 0.05; ANOVA).