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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Dev Biol. 2010 May 10;344(1):248–258. doi: 10.1016/j.ydbio.2010.05.003

Fig. 5. Tcrk is a 20E-inducible gene.

Fig. 5

(A). The expression of Tcrk in wing tissues dissected from 0 h pupae was determined using qRT-PCR after 6 and 12 hours incubation with 1 μM 20E in vitro. The expression of Tcrk (B) and TcEcR (C) in whole body samples isolated from beetles injected with malE and TcEcR dsRNA (The sequences of primers used for TcEcR dsRNA synthesis and qRT-PCR were reported by Tan and Palli, 2008). The expression levels of Tcrk and TcEcR were normalized using Tcrp49 mRNA levels as an internal control. Mean + SE of three independent experiments are shown. Asterisk indicates significant difference between treatments (p ≤ 0.05; Student t-test).