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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Dev Biol. 2010 May 10;344(1):248–258. doi: 10.1016/j.ydbio.2010.05.003

Fig. 6. Hierarchical cluster analysis of gene expression.

Fig. 6

Eighteen down-regulated genes and six up-regulated genes were selected from microarray data for the hierarchical cluster analysis. The expression data were logarithm transformed and grouped using hierarchical clustering algorithm in Gene Cluster 3.0 program. Heat-map was generated using Java Treeview program. Genes down-regulated by Tcrk RNAi were grouped into cluster 1 and 2, while genes up-regulated by Tcrk RNAi were grouped into cluster 3. The expression of cluster 1 genes is similar between 0 h and 24 h AEP. In contrast, the expression of cluster 2 genes is much higher at 0 h AEP stage when compared to the expression at 24 h AEP stage. Three genes were clustered with Tcrk, which indicates that they have similar expression pattern (enclosed in a square).