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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Mol Microbiol. 2010 Jun 1;77(3):743–754. doi: 10.1111/j.1365-2958.2010.07245.x

Figure 1.

Figure 1

TolC is expressed in a single operon with ygiBC. A. Structures of tolC-ygiBC and yjfKLMC operons. Annealing regions of primers used in Reverse Transcriptase (RT)-PCR reactions are indicated as small arrows. B. RT-PCR analysis of RNA isolated from E. coli BW25113 (WT) strain. The same RNA sample was used in all reactions. Lanes 1–3: RT-PCR reactions with forward and reverse primers that anneal to tolC-ygiB transcript (primers 1, 2 and 3 in panel A). Lane 4: RT-PCR reaction using forward and reverse primers that anneal to tolC transcript (primers 4, 5 in panel A). Lane 5 is the same reaction as in lane 4 but RT was omitted (no DNA control). C. Immunoblotting analysis of TolC in WT (BW25113), ΔtolC (GD100) and ΔygiBC (GD101) cells. 20 μg of total membrane proteins were separated on 12% SDS-PA gel. TolC was visualized using polyclonal anti-TolC antibody.