Table 5.
Intracellular (in) and excreted (out) concentrations of glutathione in the WT and ΔtolC mutants
| Medium, time of incubation | BW25113 | GD100 | GD105 | |||
|---|---|---|---|---|---|---|
| GSH+GSSG, mM | GSH+GSSG, mM | GSH+GSSG, mM | ||||
| in | out | in | out | in | out | |
| LB, 1 hr | 10.4±0.1 | NDa | 9.7±0.8 | ND | 7.6±1.9 | ND |
| LB, 12 hrs | 8.1±0.4 | ND | 8.3±0.01 | ND | 7.8±1.2 | ND |
| M9, 1 hr | 8.8±0.7 | -b | 7.1±0.3 | - | 4.2±0.3 | - |
| M9, 12 hr | 17.7±1.9 | 0.001±0.002 | 8.5±0.1 | 0.008±0.0003 | 2.4±0.2 | 0.009±0.001 |
| M9, 20 hr | 15.1±0.6 | 0.019±0.001 | 1.8±0.5 | 0.014±0.001 | 0.3±0.1 | 0.005±0.0004 |
a
ND – no data
b
- below detection levels