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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Dev Biol. 2010 May 7;344(1):94–106. doi: 10.1016/j.ydbio.2010.04.025

Fig. 2.

Fig. 2

UNC-34 localizes to apical junctions and neuronal processes. We stained embryos with anti-UNC-34 antibodies raised to the C-terminal EVH2 domain. (A-B) Pre-comma stage, wild-type embryo stained with an anti-UNC-34 antiserum (A) and DAPI (B). UNC-34 localized to the apical junctions (arrows indicate some of the junctions). (C) Two-fold and (D-H) late-stage embryos stained with the anti-UNC-34 antiserum. Late-stage embryos were dislodged from their eggshells during the staining protocol. Arrows in C, D, G and H indicate the nerve ring labeled by the antibody. Arrowheads in D indicate the apical junctions labeled by the antibody. (C, D) UNC-34 localized to axon bundles of late-stage, wild-type embryos. (E) unc-34(e951) mutants lacked all nervous system and epithelial cell staining. (F) The cell bodies but not the neuronal processes of unc-34(gm114) mutants contained UNC-34. (G-H) The nerve rings of (G) unc-34(ev561) and (H) unc-34(ev562) mutants contained reduced levels of UNC-34. scale bar is 5 μm.