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Chondroitinase ABC treatment of DSP-PG (Stains-All staining). DSP-PG was purified by a monoclonal anti-DSP antibody affinity column. Lane 1: the untreated DSP-PG migrating at ~100 - > 207 kDa. Lane 2: DSP-PG after treatment with chondroitinase ABC. Lane 3: DSP isolated from rat dentin was used as a control. Note that portions of the molecules in the DSP-PG sample co-migrated with DSP after treatment with chondroitinase ABC.
