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Superdex 75 HR 10/30 column separation of tryptic peptides from rat DSP-PG. Fractions eluted at the void volume (fractions 11-16) contained DSP-PG tryptic peptides to which GAG chains were attached. The fractions between the 2 arrows (12-14) were used for Edman degradation and MS analyses; these fractions contained peptides with amino acid sequences from the Thr162 to Arg265 region of rat DSPP (see Fig. 3A).
